Publié

Virologie

Mutations in the 3'-PPT Lead to HIV-1 Replication without Integration

Publié le - Journal of Virology

Auteurs : Clémence Richetta, Frédéric Subra, Isabelle Malet, Hervé Leh, Charlotte Charpentier, Angela Corona, Gilles Collin, Diane Descamps, Eric Deprez, Vincent Parissi, Vincent Calvez, Enzo Tramontano, Anne-Geneviève Marcelin, Olivier Delelis

Voir la publication sur HAL DOI

Our work highlights the role of HIV-1 unintegrated viral DNA in viral replication. A virus, resistant to strand-transfer inhibitors, has been selected in vitro . This virus highlights a mutation in the 3'PPT region and not in the integrase gene. This mutation modifies the reverse transcription step leading to the accumulation of 1-LTR circles and not the linear DNA. This accumulation of 1-LTR circles leads to viral replication without integration of the viral genome.