Consulter le flux RSS

Publications

Affichage des publications 21 à 30 sur 160 au total

Chimie

Triphenylamine Sensitized 8‐Dimethylaminoquinoline: An Efficient Two‐Photon Caging Group for Intracellular Delivery

Abstract Triphenylamine‐sensitized 8‐dimethylaminoquinoline (TAQ) probes showed fair two‐photon absorption and fragmentation cross sections in releasing kainate and GABA ligands. The water‐soluble PEG and TEG‐analogs allowed cell internalization and…

Delphine Rigault, Philippe Nizard, Jonathan Daniel, Mireille Blanćhard-Desce, Patrick Tauc, Hamid Dhimane, Peter Dalko

Chemistry - A European Journal -

Biologie structurale

Stimulation of ATP Hydrolysis by ssDNA Provides the Necessary Mechanochemical Energy for G4 Unfolding

The G-quadruplex (G4) is a distinct geometric and electrophysical structure compared to classical doublestranded DNA, and its stability can impede essential cellular processes such as replication, transcription, and translation. This study focuses…

Yang-Xue Dai, Xiao-Lei Duan, Wen-Tong Fu, Shan Wang, Na-Nv Liu, Hai-Hong Li, Xia Ai, Hai-Lei Guo, Cel Areny Navés, Elisabeth Bugnard, Daniel Auguin, Xi-Miao Hou, Stephane Rety, Xu-Guang Xi

Journal of Molecular Biology -

Amélioration des plantes

The catalytic triad of rice NARROW LEAF1 involves H234

NARROW LEAF1 (NAL1) exerts a multifaceted influence on leaf morphology and crop yield. Recent crystal study proposed that histidine 233 (H233) is part of the catalytic triad. Here we report that unlike suggested previously, H234 instead of H233 is a…

Ling-Yun Huang, Na-Nv Liu, Wei-Fei Chen, Xia Ai, Hai-Hong Li, Ze-Lin Zhang, Xi-Miao Hou, Philippe Fossé, Olivier Mauffret, Dong-Sheng Lei, Stephane Rety, Xu-Guang Xi

Nature Plants -

Biologie structurale

Structural insights into the N-terminal APHB domain of HrpA: mediating canonical and i-motif recognition

RNA helicases function as versatile enzymes primarily responsible for remodeling RNA secondary structures and organizing ribonucleoprotein complexes. In our study, we conducted a systematic analysis of the helicase-related activities of Escherichia…

Ben-Ge Xin, Ling-Yun Huang, Ling-Gang Yuan, Na-Nv Liu, Hai-Hong Li, Xia Ai, Dong-Sheng Lei, Xi-Miao Hou, Stephane Rety, Xu-Guang Xi

Nucleic Acids Research -

Sciences du Vivant

Direct single-cell observation of a key Escherichia coli cell-cycle oscillator

Initiation of DNA replication in Escherichia coli is coupled to cell size via the DnaA protein, whose activity is dependent on its nucleotide-bound state. However, the oscillations in DnaA activity have never been observed at the single-cell level…

Ilaria Iuliani, Gladys Mbemba, Marco Cosentino Lagomarsino, Bianca Sclavi

Science Advances -

Biologie structurale

Structural insights into the N-terminal APHB domain of HrpA: mediating canonical and i-motif recognition

RNA helicases function as versatile enzymes primarily responsible for remodeling RNA secondary str uct ures and organizing ribonucleoprotein comple x es. In our study, w e conducted a systematic analysis of the helicase-related activities of Esc…

Ben-Ge Xin, Ling-Yun Huang, Ling-Gang Yuan, Na-Nv Liu, Hai-Hong Li, Xia Ai, Dong-Sheng Lei, Xi-Miao Hou, Stephane Rety, Xu-Guang Xi

Nucleic Acids Research -

Biochimie

Nonstructural N- and C-tails of Dbp2 confer the protein full helicase activities

Human DDX5 and its yeast ortholog Dbp2 are ATP-dependent RNA helicases that play a keyrole in normal cell processes, cancer development and viral infection. The crystal structure ofthe RecA1-like domain of DDX5 is available, but the global structure…

Qin-Xia Song, Na-Nv Liu, Zhao-Xia Liu, Ying-Zi Zhang, Stephane Rety, Xi-Miao Hou, Xu-Guang Xi

Journal of Biological Chemistry -

Pharmacologie

Mechanistic target of rapamycin (mTOR) regulates self-sustained quiescence, tumor indolence, and late clinical metastasis in a Beclin-1-dependent manner

Self-sustained quiescence (SSQ) has been characterized as a stable but reversible non-proliferative cellular state that limits the cloning of cultured cancer cells. By developing refined clonogenic assays, we showed here that cancer cells in SSQ can…

Carole Nicco, Marine Thomas, Julie Guillermet-Guibert, Maryline Havard, Fanny Laurent-Tchenio, Ludivine Doridot, François Dautry, Frédéric Batteux, Thierry Tchenio

Cell Cycle -

Sciences du Vivant

The purinergic receptor P2X7 and the NLRP3 inflammasome are druggable host factors required for SARS-CoV-2 infection

Purinergic receptors and NOD-like receptor protein 3 (NLRP3) inflammasome regulate inflammation and viral infection, but their effects on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection remain poorly understood. Here, we…

Déborah Lécuyer, Roberta Nardacci, Désirée Tannous, Emie Gutierrez-Mateyron, Aurélia Deva Nathan, Frédéric Subra, Cristina Di Primio, Paola Quaranta, Vanessa Petit, Clémence Richetta, Ali Mostefa-Kara, Franca del Nonno, Laura Falasca, Romain Marlin, Pauline Maisonnasse, Julia Delahousse, Juliette Pascaud, Eric Deprez, Marie Naigeon, Nathalie Chaput, Angelo Paci, Véronique Saada, David Ghez, Xavier Mariette, Mario Costa, Mauro Pistello, Awatef Allouch, Olivier Delelis, Mauro Piacentini, Roger Le Grand, Jean-Luc Perfettini

Frontiers in Immunology -