Publications

Sciences du Vivant

Modulation of the functional interfaces between retroviral intasomes and the human nucleosome

ABSTRACT Infection by retroviruses as HIV-1 requires the stable integration of their genome into the host cells. This process needs the formation of integrase (IN)-viral DNA complexes, called intasomes, and their interaction with the target DNA…

E. Mauro, D. Lapaillerie, C. Tumiotto, C. Charlier, F. Martins, S. Sousa, M. Métifiot, P. Weigel, K. Yamatsugu, M. Kanai, Hélène Munier-Lehmann, C. Richetta, M. Maisch, J. Dutrieux, J. Batisse, M. Ruff, O. Delelis, P. Lesbats, Vincent Parissi

mBio - 2023

Immunité innée

The purinergic receptor P2X7 and the NLRP3 inflammasome are druggable host factors required for SARS-CoV-2 infection

Abstract Purinergic receptors and NOD-like receptor protein 3 (NLRP3) inflammasome regulate inflammation and viral infection, but their effects on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection remain poorly understood. Here,…

Déborah Lécuyer, Roberta Nardacci, Désirée Tannous, Emie Gutierrez-Mateyron, Aurélia Deva-Nathan, Frédéric Subra, Cristina Di Primio, Paola Quaranta, Vanessa Petit, Clémence Richetta, Ali Mostefa-Kara, Franca Del Nonno, Laura Falasca, Romain Marlin, Pauline Maisonnasse, Julia Delahousse, Juliette Pascaud, Eric Deprez, Marie Naigeon, Nathalie Chaput, Angelo Paci, Véronique Saada, David Ghez, Xavier Mariette, Mario Costa, Mauro Pistello, Awatef Allouch, Olivier Delelis, Mauro Piacentini, Roger Le Grand, Jean-Luc Perfettini

Frontiers in Immunology - 2023

Biophysique

Régulation et inhibition du facteur d'échange EPAC1, une cible thérapeutique dans l'insuffisance cardiaque

Les protéines EPAC sont des facteurs d'échange de nucléotides guanine (GEFs), directement activées par le second messager AMP cyclique (AMPc), qui activent les petites GTPases Rap à la périphérie des membranes cellulaires.La protéine EPAC1,…

Candice Sartre

Sciences du Vivant

Nonstructural N- and C-tails of Dbp2 confer the protein full helicase activities.

Human DDX5 and its yeast ortholog Dbp2 are ATP-dependent RNA helicases that play a key role in normal cell processes, cancer development, and viral infection. The crystal structure of the RecA1-like domain of DDX5 is available but the global…

Song Qx, Liu Nn, Liu Zx, Zhang Yz, Rety S, Hou Xm, Xi Xg

J. Biol. Chem. - 2023

Sciences du Vivant

Direct single cell observation of a key E. coli cell cycle oscillator

A long-standing hypothesis sees DNA replication control in E. coli as a central cell cycle os-cillator at whose core is the DnaA protein. The consensus is that the activity of the DnaA protein, which is dependent on its nucleotide bound state, is an…

Ilaria Iuliani, Gladys Mbemba, Marco Cosentino Lagomarsino, Bianca Sclavi

Biologie structurale

MDexciteR: Enhanced Sampling Molecular Dynamics by Excited Normal Modes or Principal Components Obtained from Experiments

Molecular dynamics with excited normal modes (MDeNM) is an enhanced sampling method for exploring conformational changes in proteins with minimal biases. The excitation corresponds to injecting kinetic energy along normal modes describing intrinsic…

Mauricio Costa, Paulo R Batista, Antoniel Gomes, Leonardo S Bastos, Maxime Louet, Nicolas Floquet, Paulo M Bisch, David Perahia

Journal of Chemical Theory and Computation - 2023

Chimie

Shigella IpaA mediates actin bundling through diffusible vinculin oligomers with activation imprint

Upon activation, vinculin reinforces cytoskeletal anchorage during cell adhesion. Activating ligands classically disrupt intramolecular interactions between the vinculin head and tail domains that bind to actin filaments. Here, we show that Shigella…

Cesar Valencia-Gallardo, Daniel-Isui Aguilar-Salvador, Hamed Khakzad, Benjamin Cocom-Chan, Charles Bou-Nader, Christophe Velours, Yosra Zarrouk, Christophe Le Clainche, Christian Malosse, Diogo Borges Lima, Nicole Quenech’du, Bilal Mazhar, Sami Essid, Marc Fontecave, Atef Asnacios, Julia Chamot-Rooke, Lars Malmström, Guy Tran van Nhieu

Cell Reports - 2023

Sciences du Vivant

Modulation of the functional interfaces between retroviral intasomes and the human nucleosome

ABSTRACT Infection by retroviruses as HIV-1 requires the stable integration of their genome into the host cells. This process needs the formation of integrase (IN)-viral DNA complexes, called intasomes, and their interaction with the target DNA…

E. Mauro, D. Lapaillerie, C. Tumiotto, C. Charlier, F. Martins, S. Sousa, M. Métifiot, P. Weigel, K. Yamatsugu, M. Kanai, H. Munier-Lehmann, C. Richetta, M. Maisch, J. Dutrieux, J. Batisse, M. Ruff, O. Delelis, P. Lesbats, V. Parissi

mBio - 2023

Sciences du Vivant

5-Nitro-3-(2-(4-phenylthiazol-2-yl)hydrazineylidene)indolin-2-one derivatives inhibit HIV-1 replication by a multitarget mechanism of action

Olivier Delelis

Frontiers in Cellular and Infection Microbiology - 2023

Biochimie, Biologie Moléculaire

Membranes prime the RapGEF EPAC1 to transduce cAMP signaling

EPAC1, a cAMP-activated GEF for Rap GTPases, is a major transducer of cAMP signaling and a therapeutic target in cardiac diseases. The recent discovery that cAMP is compartmentalized in membrane-proximal nanodomains challenged the current model of…

Candice Sartre, François Peurois, Marie Ley, Marie-Hélène Kryszke, Wenhua Zhang, Delphine Courilleau, Rodolphe Fischmeister, Yves Ambroise, Mahel Zeghouf, Sarah Cianférani, Yann Ferrandez, Jacqueline Cherfils

Nature Communications - 2023