Accès direct au contenu

ENS Cachan - Laboratoire de biologie et de pharmacologie appliquée (LBPA)

Version anglaise


Accueil > Equipes > Acides nucleiques

Recherche - Valorisation


Equipe « Structures et interactions des acides nucléiques », dirigée par Olivier Mauffret

  • Butovskaya E, Heddi B, Bakalar B, Richter SN, Phan AT. (2018) Major G-Quadruplex Form of HIV-1 LTR Reveals a (3 + 1) Folding Topology Containing a Stem-Loop. J. Am. Chem. Soc. 140(42):13654-13662. doi: 10.1021/jacs.8b05332.

  • Bose K, Lech CJ, Heddi B, Phan AT. (2018) High-resolution AFM structure of DNA G-wires in aqueous solution. Nat Commun. 9(1):1959. doi: 10.1038/s41467-018-04016-y.
  • Rene, B., Mauffret, O., & Fosse, P. (2018) Retroviral nucleocapsid proteins and DNA strand transfers. Biochimie Open. 7:10-25.

  • Shvadchak, V., Zgheib, S., Basta, B., Humbert, N., LangedJIK, J., Morris, MC., Ciaco, S., Maskri, O., Darlix, JL., Mauffret, O., Fosse, P. Real, E. & Mely, Y. (2018) Rationally designed peptides as efficient inhibitors of nucleic acid chaperone activity of HIV-1 nucleocapsid protein. Biochemistry. 57(30):4562-4573.
  • Mouhand A., Belfetmi A., Catala  M., Larue,V., Zargarian L. , Brachet F., Gorelick RJ., Van Heijenoort C., MirambeauG. , Barraud P., Mauffret O., Tisné C. (2018) Modulation of the HIV nucleocapsid dynamics finely tunes its RNA-binding properties during virion genesis. Nucleic Acids Res. 46:9699-9710
  • Elbahnsi A., Retureau R., Baaden M., Hartmann B., Oguey C. (2018) Holding the Nucleosome Together: A Quantitative Description of the DNA-Histone Interface in Solution. J Chem Theory Comput., 14(2):1045-1058.
  • Chen WF, Rety S, Guo HL, Dai YX, Wu WQ, Liu NN, Auguin D, Liu QW, Hou XM, Dou SX, Xi XG. (2018) Molecular Mechanistic Insights into Drosophila DHX36-Mediated G-Quadruplex Unfolding: A Structure-Based Model.  Structure. 2018 Mar 6;26(3):403-415.
  • Larue V., Catala M., Belfetmi A., Zargarian L., Mauffret O., Tisne C., (2018) 1H, 13C, and 15N Backbone and partial side-chain resonance assignments of the C-terminal domain of HIV-1 Pr55Gag encompassed in NCp15. Biomol NMR Assign. 12(1):139-143
  • Lu KY, Chen WF, Rety S, Liu NN, Wu WQ, Dai YX, Li D, Ma HY, Dou SX, Xi XG. (2018) Insights into the structural and mechanistic basis of multifunctional S. cerevisiae Pif1p helicase. Nucleic Acids Res. 2018 Feb 16;46(3):1486-1500


  • Adrian M, Winnerdy FR, Heddi B, Phan AT. (2017) Rotation of Guanine Amino Groups in G-Quadruplexes: A Probe for Local Structure and Ligand Binding. Biophys J. 113(4):775-784. doi: 10.1016/j.bpj.2017.05.053.
  • Piazza A, Cui X, Adrian M, Samazan F, Heddi B, Phan AT, Nicolas AG. (2017) Non-Canonical G-quadruplexes cause the hCEB1 minisatellite instability in Saccharomyces cerevisiae. Elife. pii: e26884. doi: 10.7554/eLife.26884.
  • Do NQ, Chung WJ, Truong THA, Heddi B, Phan AT. (2017) G-quadruplex structure of an anti-proliferative DNA sequence. Nucleic Acids Res. 45(12):7487-7493. doi: 10.1093/nar/gkx274. 
  • Zargarian L., Imeddourene AB., Gavvala K., Barthes NPF, Michel BY, Kenkack CA, Morellet N., René B., Fossé P., Burger A., Mély Y., Mauffret, O. (2017) Structural and dynamical impact of a universal fluorescent nucleoside analogue inserted into a DNA duplex. J. Phys. Chem. B. 121:11249-11261.
  • Dos Santos C, Hamadat S, Le Saux K, Newton C, Mazouni M, Zargarian L, Miro-Padovani M, Zadigue P, Delbé J, Hamma-Kourbali Y, Amiche M. (2017) Studies of the antitumor mechanism of action of dermaseptin B2, a multifunctional cationic antimicrobial peptide, reveal a partial implication of cell surface glycosaminoglycans. PLoS One. 12(8):e0182926. doi: 10.1371/journal.pone.0182926. eCollection 2017.
  • Devineau S, Zargarian L, Renault JP, Pin S. (2017) Structure and Function of Adsorbed Hemoglobin on Silica Nanoparticles: Relationship between the Adsorption Process and the Oxygen Binding Properties. Langmuir. 33(13):3241-3252.
  • Lin C., Poyer S., Zargarian L., Salpin JY., Fossé P., Mauffret O., Xie, J. (2017) Identification of acylation products in SHAPE chemistry. Bioorg. Med. Chem. Lett. 27(11):2506-2509
  • Kilin V., Gavvala K., Barthes NP., Michel BY., Shin D., Boudier C., Mauffret O., Yaschuk V., Mousli M., Ruff M., Granger F., Eiler S., Bronner C., Tor Y., Burger A., Mély, Y. (2017) Dynamics of methylated cytosine flipping by UHRF1. J. Am. Chem. Soc. 139(6):2520-2528.
  • Lin W, Ma J, Nong D, Xu C, Zhang B, Li J, Jia Q, Dou S, Ye F, Xi XG, Lu Y, Li M. (2017) Helicase Stepping Investigated with One-Nucleotide Resolution Fluorescence Resonance Energy Transfer. Phys Rev Lett. 119(13):138102.
  • Teng FY, Hou XM, Fan SH, Réty S, Dou SX, Xi XG. (2017) Escherichia coli DNA polymerase I can disrupt G-quadruplex structures during DNA replication. FEBS J. 284(23):4051-4065
  • Hou XM, Fu YB, Wu WQ, Wang L, Teng FY, Xie P, Wang PY, Xi XG (2017) Involvement of G-triplex and G-hairpin in the multi-pathway folding of human telomeric G-quadruplex. Nucleic Acids Res. 45(19):11401-11412
  • You J, Li H, Lu XM, Li W, Wang PY, Dou SX, Xi XG. (2017) Effects of monovalent cations on folding kinetics of G-quadruplexes.  Biosci Rep. 37(4).
  • Shi J, Liu NN, Yang YT, Xi XG. (2017) Purification and enzymatic characterization of Gallus gallus BLM helicase.  J Biochem. 162(3):183-191.
  • Wu WQ, Hou XM, Zhang B, Fossé P, René B, Mauffret O, Li M, Dou SX, Xi XG. (2017) Single-molecule studies reveal reciprocating of WRN helicase core along ssDNA during DNA unwinding. Sci Rep. 7:43954
  • Shi J, Chen WF, Zhang B, Fan SH, Ai X, Liu NN, Réty S, Xi XG. (2017) A helical bundle in the N-terminal domain of the BLM helicase mediates dimer and potentially hexamer formation. J Biol Chem. 292(14):5909-5920.
  • Wei XB, Zhang B, Bazeille N, Yu Y, Liu NN, René B, Mauffret O, Xi XG. (2017) A 3'-5' exonuclease activity embedded in the helicase core domain of Candida albicans Pif1 helicase. Sci Rep. 7:42865.
  • Zhao J, Zhang B, Jiang J, Liu N, Wei Q, Xi XG, Fu J. (2017) AvrXa27 binding influences unwinding of the double-stranded DNA in the UPT box. Biochem Biophys Res Commun. 484(2):390-395


  • Chen Y.§, Maskri O.§, Chaminade F., René B., Benkaroun J., Godet J., Mély Y., Mauffret O., Fossé P. (2016) Structural insights into the HIV-1 minus-strand strong-stop DNA. J. Biol. Chem.,291(7):3468-3482.
  • Ammar FF, Hobaika Z, Abdel-Azeim S, Zargarian L, Maroun RG, Fermandjian S. (2016) A targeted DNA substrate mechanism for the inhibition of HIV-1 integrase by inhibitors with antiretroviral activity. FEBS Open Bio. 6(4):234-50.
  • Belfetmi A., Zargarian L., Tisné C., Sleiman D., Morellet N., Lescop E., Maskri O., René B., Mély Y., Fossé P., Mauffret, O. (2016) Insights into the mechanisms of RNA secondary structure destabilization by the HIV-1-nucleocapsid protein. RNA. 22(4):506-517.
  • Imeddourene AB., Xu X., Zargarian L., Oguey C., Foloppe N., Mauffret O., Hartmann, B. (2016) The intrinsic mechanics of B-DNA in solution characterized by NMR. Nucleic Acids Res. 44(7):3432-3447.
  • Zhang YW, Nong DG, Dou SX, Li W, Yan Y, Xi XG, Xu CH, Li M. (2016) Iterative homology checking and non-uniform stepping during RecA-mediated strand exchange. Biochem Biophys Res Commun. 478(3):1153-7.
  • Zhang B, Wu WQ, Liu NN, Duan XL, Li M, Dou SX, Hou XM, Xi XG. (2016) G-quadruplex and G-rich sequence stimulate Pif1p-catalyzed downstream duplex DNA unwinding through reducing waiting time at ss/dsDNA junction. Nucleic Acids Res. 44(17):8385-94.
  • Qin W, Bazeille N, Henry E, Zhang B, Deprez E, Xi XG. (2016) Mechanistic insight into cadmium-induced inactivation of the Bloom protein Sci Rep. 6:26225.
  • Li JH, Lin WX, Zhang B, Nong DG, Ju HP, Ma JB, Xu CH, Ye FF, Xi XG, Li M, Lu Y, Dou SX. (2016) Pif1 is a force-regulated helicase. Nucleic Acids Res. 44(9):4330-9.
  • Chen WF, Dai YX, Duan XL, Liu NN, Shi W, Li N, Li M, Dou SX, Dong YH, Rety S, Xi XG. (2016) Crystal structures of the BsPif1 helicase reveal that a major movement of the 2B SH3 domain is required for DNA unwinding. Nucleic Acids Res. 2016 Apr 7;44(6):2949-61.
  • Hatakeyama A, Hartmann B, Travers A, Nogues C, Buckle. (2016) High-resolution biophysical analysis of the dynamics of nucleosome formation. Sci Rep., 6:27337




  • Foloppe N., Guéroult M., Hartmann B. (2013) Simulating DNA by molecular dynamics: aims, methods and validation. Methods Mol Biol. 924: 445-68.


  • Bazzi, A., Zargarian, L., Chaminade, F., De Rocquigny, H., René, B., Mély, Y., Fossé, P. & Mauffret, O. (2012) Intrinsic nucleic acids dynamics modulates HIV-1 nucleocapsid protein binding to its targets. PLoS ONE , 7(6):e41704
  • Xu Y.N., Bazeille N., Ding X.Y., Lu X.M., Wang P.Y., Bugnard E., Grondin V.,  Dou S.X., Xi X.G. (2012) Multimeric BLM is dissociated upon ATP hydrolysis and functions as monomers in resolving DNA structures. Nucleic Acids Res. 40(19):9802-14.
  • Guéroult M., Boittin O., Mauffret O., Etchebest E., Hartmann B. (2012) Mg2+ in the major groove modulates B-DNA structure and dynamics. PLoS ONE 2012 7(7):e41704.


  • Bazzi A., Zargarian L., Chaminade F., Boudier C., De Rocquigny H., René B., Mély Y., Fossé P., and Mauffret O. (2011) Structural insights into the cTAR DNA recognition by the HIV-1 nucleocapsid protein: role of sugar deoxyriboses in the binding polarity of NC. Nucleic Acids Res., 39(9):3903-3916.

  • Kaushik, S., Kaushik, M., Svinarchuk, F., Malvy, M., Fermandjian, S., & Kukrekti, S. (2011) Presence of divalent cation is not mandatory for the formation of intramolecular Purine-Motif Triplex containing human-cjun protooncogene target., Biochemistry, 50(19):4132-4142.
  • Darlix, JL., Godet, J., Ivanyi-Nagy, R., Fossé, P., Mauffret, O. & Mély, Y. (2011) Flexible nature and specific functions of the HIV-1 nucleocapsid protein. J.Mol.Biol., 410(4):565-581.

  • Kanevsky, I., Chaminade, F., Chen, YY., Godet, J., René, B., Darlix, JL., Mély, Y., Mauffret, O., Fossé, P. (2011) Structural determinants of TAR DNA annealing in the absence and presence of HIV-1 nucleocapsid protein. Nucleic Acids Res., 39(18):8148-62.

  • KJ. M. Zaal1, E.Reid, K. Mousavi, T. Zhang, A. Mehta, E. Bugnard, V. Sartorelli, E. Ralston (2011) Who Needs Microtubules? Myogenic Reorganization of MTOC, Golgi Complex and ER Exit Sites Persists Despite Lack of Normal Microtubule Tracks. PLoS ONE, 6 (12), e29057.
  • Wang Y, Xiao H, Wu N, Shi H, Xu H, Zhou L, Xi XG, Wang T, Wang X.(2011) Characterization of the antiviral activity for influenza viruses M1 zinc finger peptides. Curr Microbiol.62(1):126-32.