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ENS Cachan - Laboratoire de biologie et de pharmacologie appliquée (LBPA)

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Recent publications

Equipe d'Eric Deprez


Thierry E, Deprez E, Delelis O. Different Pathways Leading to Integrase Inhibitors Resistance. Front Microbiol. 2017 Jan 11;7:2165. Review.


Stranix BR, Wu JJ, Milot G, Beaulieu F, Bouchard JE, Gouveia K, Forte A, Garde S, Wang Z, Mouscadet JF, Delelis O, Xiao Y. « Pyridoxine hydroxamic acids as novel HIV-integrase inhibitors. » Bioorg Med Chem Lett. 2016 Feb 15;26(4):1233-6.

Corona A, di Leva FS, Rigogliuso G, Pescatori L, Madia VN, Subra F, Delelis O, Esposito F, Cadeddu M, Costi R, Cosconati S, Novellino E, di Santo R, Tramontano E. New insights into the interaction between pyrrolyl diketoacids and HIV-1 integrase active site and comparison with RNase H. Antiviral Res. 2016 Oct;134:236-243.

Qin W, Bazeille N, Henry E, Zhang B, Deprez E, Xi XG. Mechanistic insight into cadmium-induced inactivation of the Bloom protein. Sci Rep. 2016 May 19;6:26225.

Thierry S, Thierry E, Subra F, Deprez E, Leh H, Bury-Moné S, Delelis O. Opposite transcriptional regulation of integrated vs unintegrated HIV genomes by the NF-B pathway. Sci Rep. 2016 May 11;6:25678.

Chennoufi R, Bougherara H, Gagey-Eilstein N, Dumat B, Henry E, Subra F, Bury-Moné S, Mahuteau-Betzer F, Tauc P, Teulade-Fichou MP, Deprez E. Mitochondria-targeted Triphenylamine Derivatives Activatable by Two-Photon Excitation for Triggering and Imaging Cell Apoptosis. Sci Rep. 2016 Mar 7;6:21458.

El Shafey N, Guesnon M, Simon F, Deprez E, Cosette J, Stockholm D, Scherman D, Bigey P, Kichler A. Inhibition of the myostatin/Smad signaling pathway by short decorin-derived peptides. Exp Cell Res. 2016 Feb 15;341(2):187-95.


Thierry S, Benleulmi MS, Sinzelle L, Thierry E, Calmels C, Chaignepain S, Waffo-Teguo P, Merillon JM, Budke B, Pasquet JM, Litvak S, Ciuffi A, Sung P, Connell P, Hauber I, Hauber J, Andreola ML, Delelis O, Parissi V. Dual and Opposite Effects of hRAD51 Chemical Modulation on HIV-1 Integration. Chem Biol. 2015 Jun 18;22(6):712-23.

Liu NN, Duan XL, Ai X, Yang YT, Li M, Dou SX, Rety S, Deprez E, Xi XG. The Bacteroides sp. 3_1_23 Pif1 protein is a multifunctional helicase. Nucleic Acids Res. 2015 Oct 15;43(18):8942-54.

Malet I, Thierry E, Wirden M, Lebourgeois S, Subra F, Katlama C, Deprez E, Calvez V, Marcelin AG, Delelis O. Combination of two pathways involved in raltegravir resistance confers dolutegravir resistance. J Antimicrob Chemother. 2015 Oct;70(10):2870-80.

Thierry S, Munir S, Thierry E, Subra F, Leh H, Zamborlini A, Saenz D, Levy DN, Lesbats P, Saïb A, Parissi V, Poeschla E, Deprez E, Delelis O. Integrase inhibitor reversal dynamics indicate unintegrated HIV-1 dna initiate de novo integration. Retrovirology. 2015 Mar 12;12:24.

Munir S, Thierry E, Malet I, Subra F, Calvez V, Marcelin AG, Deprez E, Delelis O. G118R and F121Y mutations identified in patients failing raltegravir treatment confer dolutegravir resistance. J Antimicrob Chemother. 2015 Mar;70(3):739-49.

Qamar Saeed M, Dufour N, Bartholmae C, Sieranska U, Knopf M, Thierry E, Thierry S, Delelis O, Grandchamp N, Pilet H, Ravassard P, Massonneau J, Pflumio F, von Kalle C, Lachapelle F, Bemelmans AP, Schmidt M, Serguera C. Comparison Between Several Integrase-defective Lentiviral Vectors Reveals Increased Integration of an HIV Vector Bearing a D167H Mutant. Mol Ther Nucleic Acids. 2014 Dec 2;3:e213.

Mbemba G, Henry E, Delelis O, Bouger MC, Buckle M, Mouscadet JF, Hazan U, Leh H, Bury-Moné S. Biochemical properties of the xenotropic murine leukemia virus-related virus integrase. Biochimie. 2014 Dec;107 Pt B:300-9.


Corona A, Di Leva FS, Thierry S, Pescatori L, Cuzzucoli Crucitti G, Subra F, Delelis O, Esposito F, Rigogliuso G, Costi R, Cosconati S, Novellino E, Di Santo R, Tramontano E. Identification of highly conserved residues involved in inhibition of HIV-1 RNase H function by Diketo acid derivatives. Antimicrob Agents Chemother. 2014 Oct;58(10):6101-10.

Rouaud F, Romero-Perez M, Wang H, Lobysheva I, Ramassamy B, Henry E, Tauc P, Giacchero D, Boucher JL, Deprez E, Rocchi S, Slama-Schwok A. Regulation of NADPH-dependent Nitric Oxide and reactive oxygen species signalling in endothelial and melanoma cells by a photoactive NADPH analogue. Oncotarget. 2014 Nov 15;5(21):10650-64.

Malet I, Gimferrer Arriaga L, Artese A, Costa G, Parrotta L, Alcaro S, Delelis O, Tmeizeh A, Katlama C, Valantin MA, Ceccherini-Silberstein F, Calvez V, Marcelin AG. New raltegravir resistance pathways induce broad cross-resistance to all currently used integrase inhibitors. J Antimicrob Chemother. 2014 Aug;69(8):2118-22.


Pflieger A, Waffo Teguo P, Papastamoulis Y, Chaignepain S, Subra F, Munir S, Delelis O, Lesbats P, Calmels C, Andreola ML, Merillon JM, Auge-Gouillou C, Parissi V. Natural stilbenoids isolated from grapevine exhibiting inhibitory effects against HIV-1 integrase and eukaryote MOS1 transposase in vitro activities. PLoS One. 2013 Nov 28;8(11):e81184.

Le Rouzic E, Bonnard D, Chasset S, Bruneau JM, Chevreuil F, Le Strat F, Nguyen J, Beauvoir R, Amadori C, Brias J, Vomscheid S, Eiler S, Lévy N, Delelis O, Deprez E, Saïb A, Zamborlini A, Emiliani S, Ruff M, Ledoussal B, Moreau F, Benarous R. Dual inhibition of HIV-1 replication by integrase-LEDGF allosteric inhibitors is predominant at the post-integration stage. Retrovirology. 2013 Nov 21;10:144.

Munir S, Thierry S, Subra F, Deprez E, Delelis O. Quantitative analysis of the time-course of viral DNA forms during the HIV-1 life cycle. Retrovirology. 2013 Aug 13;10:87.

Han YS, Xiao WL, Quashie PK, Mesplède T, Xu H, Deprez E, Delelis O, Pu JX, Sun HD, Wainberg MA. Development of a fluorescence-based HIV-1 integrase DNA binding assay for identification of novel HIV-1 integrase inhibitors. Antiviral Res. 2013 Jun;98(3):441-8.

Manic G, Maurin-Marlin A, Laurent F, Vitale I, Thierry S, Delelis O, Dessen P, Vincendeau M, Leib-Mösch C, Hazan U, Mouscadet JF, Bury-Moné S. Impact of the Ku complex on HIV-1 expression and latency. PLoS One. 2013 Jul 29;8(7):e69691

Brahimi-Adouane S, Bachet JB, Tabone-Eglinger S, Subra F, Capron C, Blay JY, Emile JF. Effects of endoplasmic reticulum stressors on maturation and signaling of hemizygous and heterozygous wild-type and mutant forms of KIT. Mol Oncol. 2013 Jun;7(3):323-33.

Hamdi FS, Français O, Subra F, Dufour-Gergam E, Le Pioufle B. « Microarray of non-connected gold pads used as high density electric traps for parallelized pairing and fusion of cells. Biomicrofluidics. 2013 Jul 3;7(4):44101.

Saleh R, Wedeh G, Herrmann H, Bibi S, Cerny-Reiterer S, Sadovnik I, Blatt K, Hadzijusufovic E, Jeanningros S, Blanc C, Legarff-Tavernier M, Chapiro E, Nguyen-Khac F, Subra F, Bonnemye P, Dubreuil P, Desplat V, Merle-Béral H, Willmann M, Rülicke T, Valent P, Arock M. A new human mast cell line expressing a functional IgE receptor converts to tumorigenic growth by KIT D816V transfection. Blood. 2014 Jul 3;124(1):111-20.


Li J, Henry E, Wang L, Delelis O, Wang H, Simon F, Tauc P, Brochon JC, Zhao Y, Deprez E. Comparative study of the fatty acid binding process of a new FABP from Cherax quadricarinatus by fluorescence intensity, lifetime and anisotropy. PLoS One. 2012;7(12):e51079.

Li Y, Wang H, Tarus B, Perez MR, Morellato L, Henry E, Berka V, Tsai AL, Ramassamy B, Dhimane H, Dessy C, Tauc P, Boucher JL, Deprez E, Slama-Schwok A. Rational design of a fluorescent NADPH derivative imaging constitutive nitric-oxide synthases upon two-photon excitation. Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12526-31.

Nogues C, Leh H, Lautru J, Delelis O, Buckle M. Efficient antifouling surface for quantitative surface plasmon resonance based biosensor analysis. PLoS One. 2012;7(9):e44287.

Zamborlini A, Coiffic A, Beauclair G, Delelis O, Paris J, Koh Y, Magne F, Giron ML, Tobaly-Tapiero J, Deprez E, Emiliani S, Engelman A, de Thé H, Saïb A. Impairment of human immunodeficiency virus type-1 integrase SUMOylation correlates with an early replication defect. J Biol Chem. 2011 Jun 10;286(23):21013-22.  

Manic G, Maurin-Marlin A, Galluzzi L, Subra F, Mouscadet JF, Bury-Moné S. 3' self-inactivating long terminal repeat inserts for the modulation of transgene expression from lentiviral vectors. Hum Gene Ther Methods. 2012 Apr;23(2):84-97.

Gasecka A, Tauc P, Lewit-Bentley A, Brasselet S. Investigation of molecular and protein crystals by three photon polarization resolved microscopy. Phys Rev Lett. 2012 Jun 29;108(26):263901.


Zamborlini A., Coiffic A., Beauclair G., Delelis O., Paris J., Koh Y., Magne F., Giron M.L., Tobaly-Tapiero J., Deprez E., Emiliani S., Engelman A., de The H. & Saib A. Impairment of human immunodeficiency virus type-1 integrase SUMOylation correlates with an early replication defect. J. Biol. Chem. (2011) 286, 21013-21022

Mouscadet J-F., Deprez E., Desmaele D. & d'Angelo J. Development of styrylquinoline integrase inhibors Dans « HIV-1 Integrase: Mechanism of Action and Inhibitor Design » (Nouri Neamati, Binghe Wang - Editeur Wiley-Interscience), 2011, Chapitre d'ouvrage.

Charpentier C, Roquebert B, Delelis O, Larrouy L, Matheron S, Tubiana R, Karmochkine M, Duval X, Chêne G, Storto A, Collin G, Bénard A, Damond F, Mouscadet JF, Brun-Vézinet F, Descamps D. Hot spots of integrase genotypic changes leading to HIV-2 resistance to raltegravir. French ANRS HIV-2 Cohort (ANRS CO 05 VIH-2). Antimicrob Agents Chemother. (2011) 55, 1293-5.

Olesiak-Baska J, Mojzisova H, Chauvat D, Zielinski M, Matczyszyn K, Tauc P, Zyss J. Liquid crystal phases of DNA: Evaluation of DNA organization by two-photon fluorescence microscopy and polarization analysis.  Biopolymers (2011) 95, 365-375


Carayon K., Leh H., Henry E., Simon F., Mouscadet J-F. & Deprez E. A cooperative and specific DNA-binding mode of HIV-1 integrase depends on the nature of the metallic cofactor and involves the zinc-containing N-terminal domain.Nucleic Acids Research (2010) 38, 3692-3708.

Xi X-G & Deprez E. Monitoring helicase-catalyzed DNA unwinding by fluorescence anisotropy and fluorescence cross-correlation spectroscopy.Methods (2010) 51, 289-294.

Zamborlini A, Renault N, Saïb A, Delelis O. Early reverse transcription is essential for productive foamy virus infection. PLoS One. (2010) 5:e11023.

Li N., Henry E., Guiot E., Rigolet P., Brochon J-C., Xi X-G. & Deprez E. Multiple Escherichia coli helicase monomers cooperate to unwind long DNA substrates: A fluorescence cross-correlation spectroscopy study. J. Biol. Chem. (2010) 285, 6922-6936.

Li C., Henry E., Kumar Mani N., Tang J., Brochon J-C., Deprez E.* & Xie J.* Click Chemistry to Fluorescent Amino Esters: Synthesis and Spectroscopic Studies.Eur. J. Org. Chem. (2010) 2010, 2395-2405.

Delelis O., Thierry S., Subra F., Simon F., Malet I., Alloui C., Sayon S., Calvez V., Deprez E., Marcelin A-G., Tchertanov L. & Mouscadet J-F. Impact of Y143 HIV-1 integrase mutations on resistance to Raltegravir in vitro and in vivo. Antimicrob Agents Chemother (2010) 54, 491-501.

Perfettini JL, Nardacci R, Séror C, Raza SQ, Sepe S, Saïdi H, Brottes F, Amendola A, Subra F, Del Nonno F, Chessa L, D'Incecco A, Gougeon ML, Piacentini M, Kroemer G. 53BP1 represses mitotic catastrophe in syncytia elicited by the HIV-1 envelope. Cell Death Differ. (2010) 17, 811-20.


Faklaris O., Joshi V., Irinopoulou T., Tauc P., Sennour M., Girard H., Gesset C., Arnault J-C., Thorel A., Boudou J-P., Curmi P.A. & Treussart F. Photoluminescent nanodiamond nanoparticles for cell labelling: study of the uptake mechanism in mammalian cells.ACS Nano (2009) 3, 3955-3962.

Delelis O., Malet I., Li N., Tchertanov L., Calvez V., Marcelin A-G., Subra F., Deprez E. & Mouscadet  J-F. The G140S Mutation in HIV Integrases from Raltegravir-Resistant Patients Rescues Catalytic Defect due to the Resistance Q148H Mutation. Nucleic Acids Res. (2009) 37, 1193-1201.

Beaumont E., Lambry J-C., Blanchard-Desce M., Martasek P., Panda SP., van Faassen E.E., Brochon J-C., Deprez E. & Slama-Schwok A. NO formation by neuronal NO-synthase can be controlled by ultrafast electron injection from a nanotrigger. Chembiochem (2009) 10, 690-701.

Bougherara H, Subra F, Crépin R, Tauc P, Auclair C & Poul MA. The aberrant localization of oncogenic Kit tyrosine kinase receptor mutants is reversed on specific inhibitory treatment. Molecular Cancer Research (2009) 7, 1525-1533.

Yakovlev AV , Zhang F, Zulqurnain A, Azhar-Zahoor A, Luccardini C, Gaillard S, Mallet JM, Tauc P, Brochon JC, Parak WJ, Feltz A & Oheim M. Wrapping nanocrystals with an amphiphilic polymer preloaded with fixed amounts of  fluorophore generates FRET-based nanoprobes with a controlled donor/acceptor ratio. Langmuir (2009) 25, 3232-3239.

Maïga AI, Malet I, Soulie C, Derache A, Koita V, Amellal B, Tchertanov L, Delelis O, Morand-Joubert L, Mouscadet JF, Murphy R, Cissé M, Katlama C, Calvez V, Marcelin AG. Genetic barriers for integrase inhibitor drug resistance in HIV type-1 B and CRF02_AG subtypes. Antivir Ther. (2009) 14, 123-9.

Malet I, Delelis O, Soulie C, Wirden M, Tchertanov L, Mottaz P, Peytavin G, Katlama C, Mouscadet JF, Calvez V, Marcelin AG. Quasispecies variant dynamics during emergence of resistance to raltegravir in HIV-1-infected patients. J. Antimicrob Chemother. (2009) 63, 795-804.


Delelis O., Carayon K., Guiot E., Leh H., Tauc P., Brochon J-C., Mouscadet J-F. & Deprez E. Insight into the integrase-DNA recognition mechanism: A specific DNA-binding mode revealed by an enzymatically labeled integrase. J. Biol. Chem. (2008) 283, 27838-27849.

Delelis O., Carayon K., Saib A., Deprez E. & Mouscadet J-F. Integrase and integration: Biochemical activities of HIV-1 Integrase. Retrovirology (2008) 5, 114.

Tobaly-Tapiero J, Bittoun P, Lehmann-Che J, Delelis O, Giron ML, de Thé H, Saïb A. Chromatin tethering of incoming foamy virus by the structural Gag protein. Traffic (2008) 9, 1717-27.

Bordeau G, Allain C, Lartia R, Granhzhan A, Faurel E, Fiorini-Debuisschert C, Charra F, Metge G, Tauc P & Teulade-Fichou MP. Novel fluorophores with two-photon absorption properties for specific labelling of DNA. Chemistry of Nucl. Ac. Components (2008) 10, 234-38.

Malet I, Delelis O, Valantin MA, Montes B, Soulie C, Wirden M, Tchertanov L, Peytavin G, Reynes J, Mouscadet JF, Katlama C, Calvez V, Marcelin AG. Mutations associated with failure of raltegravir treatment affect integrase sensitivity to the inhibitor in vitro. Antimicrob Agents Chemother. (2008) 52, 1351-8.

Perfettini JL, Nardacci R, Bourouba M, Subra F, Gros L, Séror C, Manic G, Rosselli F, Amendola A, Masdehors P, Chessa L, Novelli G, Ojcius DM, Siwicki JK, Chechlinska M, Auclair C, Regueiro JR, de Thé H, Gougeon ML, Piacentini M, Kroemer G. Critical involvement of the ATM-dependent DNA damage response in the apoptotic demise of HIV-1-elicited syncytia. PLoS One. (2008) 3:e2458.


Delelis O., Parissi V., Leh H., Mbemba G., Petit C., Sonigo P., Deprez E. & Mouscadet J-F. Efficient and specific internal cleavage of a retroviral palindromic DNA sequence by tetrameric HIV-1 integrase. PLoS One (2007) 2:e608.

Allain C, Schmidt F, Lartia R, Bordeau G, Fiorini-Debuisschert C, Charra F, Tauc P, Teulade-Fichou MP. Vinyl-pyridinium triphenylamines: novel far-red emitters with high photostability and twophoton absorption properties for staining DNA. Chembiochem. (2007) 8, 424- 433.

Ishow E, Brosseau A, Clavier G, Nakatani K, Pansu RB, Vachon JJ, Tauc P, Chauvat D, Mendonça CR, Piovesan E. Two-photon fluorescent holographic rewritable micropatterning. J. Am. Chem. Soc. (2007) 129, 8970-1.

Fernandez-Argüelles MT, Yakovlev A, Sperling RA, Luccardini C, Gaillard S, Medel AS, Mallet JM, Brochon JC, Feltz A, Oheim M, Parak WJ. Synthesis and characterization of polymer-coated quantum dots with integrated acceptor dyes as FRET-based nanoprobes. Nano Lett. (2007) 7, 2613-7.


Guiot E., Carayon K., Delelis O., Simon F., Tauc P., Zubin E., Gottikh M., Mouscadet J-F., Brochon J-C. & Deprez E. Relationship between the oligomeric status of HIV-1 Integrase on DNA and enzymatic activity. J. Biol. Chem. (2006) 281, 22707-22719.

Ishchenko A.A., Deprez E., Maksimenko A., Brochon, J-C., Tauc P. & Saparbaev M.K. Uncoupling of the base excision and nucleotide incision repair pathways reveals their respective biological roles. Proc. Natl. Acad. Sci. U.S.A (2006) 103, 2564-2569.

Smolov M., Gottikh M., Tashlitskii V., Korolev S., Demidyuk I., Brochon J-C., Mouscadet J-F. & Deprez E. Kinetic study of the HIV-1 DNA 3'-end processing: single-turnover property of integrase. FEBS Journal (2006) 273, 1137-1151.

Agapkina I., Smolov M., Barbe S., Zubin E., Zatsepin T., Deprez E., Le Bret M., Mouscadet J-F. & Gottikh M. Probing of HIV-1 integrase-DNA interactions using novel analogs of viral DNA. J. Biol. Chem. (2006) 281, 11530-11540.

Maroun M, Delelis O, Coadou G, Bader T, Ségéral E, Mbemba G, Petit C, Sonigo P, Rain JC, Mouscadet JF, Benarous R, Emiliani S. Inhibition of early steps of HIV-1 replication by SNF5/Ini1. J Biol Chem. (2006) 281, 22736-43.